Immunology and Diabetes - Senior Research Staff - Dr Stuart Mannering
Dr Stuart Mannering
Laboratory Head, Human T cell Laboratory
Education and Professional Experience
1988 BSc Canterbury University, Christchurch, New Zealand
1994 M.Sc, University of Otago, Dunedin, New Zealand
1998 PhD, University of Otago, Dunedin, New Zealand
1998 Postdoctoral Fellow, University of Melbourne, Australia
2001 Postdoctoral Fellow, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Achievements
2005 JDRF Advanced Postdoctoral Fellowship
2005 Immunology of Diabetes Society Travel Award
2009 JDRF Transition Award
1997 Presentation award European Group for Blood and Bone Marrow Transplantation
2005 Founder of the Melbourne Human Immunology Group
2004- Member of the Immunology of Diabetes Society T cell workshop
2010 Member of the Editorial Board for Immunoassay and Immunochemistry
2010 Member of the Editorial Board for Clinical and Developmental Immunology
Research Interests
My research interest is to understand how and why human T cells cause autoimmune diseases. Specifically, I am interested in understanding what antigens autoreactive T cells see and why those structures are recognized by auto-pathogenic T cells. How a T cell responds to a particular antigen changes the resulting immune response, hence I am interested in understanding what affects T cell function and in developing assays to detect autoantigen-specific T cells and analyzing their function in vitro.
Selected Publications
- Dromey JA, Lee BH, Yu H, Young HE, Thearle DJ, Jensen KP, Mannering SI, Harrison LC. Generation and expansion of regulatory human CD4+ T-cell clones specific for pancreatic islet autoantigens Journal of Autoimmunity (in press)
- Tye-Din JA, Stewart JA, Dromey JA, Beissbarth T, van Heel DA, Tatham A, Henderson K, Mannering SI, Gianfrani C, Jewell DP, Hill AVS, McCluskey J, Rossjohn J and Anderson RP Design of peptide-based immunotherapy and diagnostics for celiac disease based upon comprehensive, quantitative mapping of T-cell epitopes in gluten (2010) Science Translational Medicine 2(4) 41-51
- Moon HC, Joffe M, Thomas HE, Kay TW, Mannering SI. A method for extracting tissue proteins for use in lymphocyte function assays (2010). Journal of Immunological Methods 359, 56-60
- Mallone R, Mannering SI, Brooks-Worrell BM, Cilio CM, Wong FS and Schloot NC Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T-cell responses Cinical and Experimental Immunology (accepted August 16th 2010
- Mannering SI, Wong FS, Durinovic-Belló I, Brooks-Worrell B, Tree TI, Cilio CM, Schloot NC and Mallone R. Current approaches to measuring human islet-antigen specific T cell function in type 1 dibetes Cinical and Experimental Immunologl (accepted July 7th 2010) (invited Review)
- Mannering SI, Pang SH, Williamson NA, Naselli G, Reynolds EC, O'Brien-Simpson NM, Purcell AW, Harrison LC, The A-chain of insulin is a hot-spot for CD4+ T cell epitopes in human type 1 diabetes. Clinical and experimental immunology 2009; 156: 226-31.
- Campbell PD, Estella E, Dudek NL, Jhala G, Thomas HE, Kay TW, Mannering SI. Cytotoxic T-lymphocyte-mediated killing of human pancreatic islet cells in vitro. Human Immunology 2008; 69: 543-551
- Henderson KN, Tye-Din JA, Reid HH, Chen Z, Borg NA, Beissbarth T, Tatham A, Mannering SI, Purcell AW, Dudek NL, van Heel DA, McCluskey J, Rossjohn J, Anderson RP. A Structural and Immunological Basis for the Role of Human Leukocyte Antigen DQ8 in Celiac Disease. Immunity 2007; 27:23-34
- Mannering SI, Harrison LC, Williamson NA, Morris JS, Thearle DJ, Jensen KP, Kay TW, Rossjohn J, Falk BA, Nepom GT, Purcell AW. The insulin A-chain epitope recognized by human T cells is posttranslationally modified. Journal of Experimental Medicine 2005; 202: 1191-7
- Mannering SI, Dromey JA, Morris JS, Thearle DJ, Jensen KP, Harrison LC. An efficient method for cloning human autoantigen-specific T cells. Journal of Immunological Methods 2005; 298:83-92