Stem Cell Regulation - Senior Research Staff - Dr Carl Walkley

Dr Carl Walkley

Research Fellow and Joint Head, Stem Cell Regulation Lab, SVI
Phillip Desbrow Senior Research Fellow of the Leukaemia Foundation

Education and Professional Experience

1997       BPharm, University of South Australia
1999       BPharm (Hons), University of South Australia
2003       PhD, Peter MacCallum Cancer Centre, University of Melbourne
2004-07  Post-doctoral Fellow, Dana-Farber Cancer Institute, Boston, USA
2008       Senior Research Officer, St. Vincent’s Institute
2008       Honorary Fellow, Department of Medicine, University of Melbourne

Achievements

2000-03    Australian Postgraduate Award
2006-09    Special Fellow Award, Leukemia & Lymphoma Society USA
2009-12    NHMRC Career Development Award Level 1
2010-14    Leukaemia Foundation Philip Desbrow Senior Research Fellow

Research Interests

My research focuses on two major areas: understanding the role of cell cycle control in fate decisions in the haemopoietic system and in generating models of human cancer.

We use haemopoiesis as the model system to understand the role of cell cycle regulation in cell fate decisions, with a particular focus on the partitioning of self-renewal and differentiation fates of haemopoietic stem cells and to understand the coupling of terminal cell cycle exit with differentiation of erythroid and myeloid cells.

We are also interested in generating faithful models of human cancer, with our current efforts focused around models of osteosarcoma, the most common tumour of bone.

Selected Publications

  1. WALKLEY, C.R., Fero, M.L., Chien, W-M., Purton, L.E.* & McArthur, G.A.*. (2005) Negative Cell Cycle Regulators Co-Operatively Control Self-Renewal and Differentiation of Haemopoietic Stem Cells. Nat Cell Biol 7,172-8.
  2. Purton, L.E., Dworkin, S., Haines, G., WALKLEY, C.R., Fabb, S.A., Collins S.J. & Chambon, P. (2006) RARγ is Critical for Maintaining a Balance between Hematopoietic Stem Cell Self-Renewal and Differentiation. J Exp Med 203,1283-93.
  3. WALKLEY, C.R., Shea, J.M., Sims, N.A., Purton, L.E. & Orkin, S.H. (2007) Rb Regulates Interactions Between Hematopoietic Stem Cells and their Bone Marrow Microenvironment. Cell 129, 1081-1095.
  4. WALKLEY, C.R.*, Haines Olsen, G.*, Dworkin, S., Fabb, S.A., Swann, J., McArthur, G.A., Westmoreland, S.V., Chambon, P., Scadden, D.T. & Purton, L.E. (2007) A Microenvironment-Induced Myeloproliferative Syndrome Caused by Retinoic Acid Receptor γ Deficiency. Cell 129, 1097-1110.
  5. North, T.E., Goessling, W., WALKLEY, C.R., Lengerke, C., Kopani, K.R., Lord, A.M., Weber, G., Venezia, T., Jang, I.H., Grosser, T., FitzGerald, G.A., Daley, G.Q., Orkin, S.H. & Zon, L.I. (2007) Prostaglandin E2 Regulates Vertebrate Haematopoietic Stem Cell Homeostasis. Nature 447, 1007-1011.
  6. Sankaran, V.G., Orkin, S.H. & WALKLEY, C.R. (2008) Rb Intrinsically Promotes Erythropoiesis by Coupling Cell Cycle Exit with Mitochondrial Biogenesis. Genes & Dev 22, 463-475.
  7. Walkley, C.R., Qudsi, R., Sankaran, V.G., Perry, J.A., Gostissa, M., Roth, S.I., Rodda, S.J., Snay, E., Dunning, P., Fahey, F.H., Alt, F.W., McMahon, A.P. & Orkin, S.H. (2008) Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease. Genes & Dev 22, 1662-1676.
  8. Hartner, J.C., WALKLEY, C.R., Lu, J. & Orkin. S.H. (2009) ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling. Nature Imm 10, 109-115.
  9. Sankaran, V.G., Xu, J., Ragoczy, T., Ippolito, G.C., Walkley, C.R., Maika, S.D., Fujiwara, Y., Ito, M., Groudine, M., Bender, M.A., Tucker, P.W. & Orkin, S.H. (2009) Developmental and species-divergent globin switching are driven by BCL11A. Nature 460, 1093-1097
  10. Janeway, K.A. & Walkley, C.R. (2010) Modeling human osteosarcoma in the mouse: From bedside to bench. Bone 47, 859-865.