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Regulation of Haemtopoietic stem cell function by circular RNAs

Regulation of Haemtopoietic stem cell function by circular RNAs

PhD/Honours project

Haematopoietic stem cells (HSCs) are specialized multipotential cells found in the bone marrow, from which all immune cells are derived. As stem cells, they must be able to self-renew as well as differentiate. The immune system is broadly divided into the myeloid branch (monocytes, granulocytes, dendritic cells, etc) and lymphoid branch (T cells, B cells, innate lymphocytes, etc). Differentiation along the myeloid versus lymphoid branches bifurcates at an early stage in haematopoiesis, and we are interested in the role that RNA pathways play. Circular RNAs are non-coding RNAs that are derived from back-splicing of messenger RNAs. They are thought to have important regulatory functions with a number shown to function as sponges for microRNAs. This project will investigate the potential role of circRNAs in regulating the differentiation of HSCs down the myeloid branch. The expression of circRNAs will by mapped by constructing RNaseA-resistant RNAseq libraries, and function will be determined by knockdown.

 

Supervised by:

  • Associate Professor Mark Chong
  • Disease Focus:

  • Cancer
  • Research Unit:

  • Genomics & immunology