Head, Vascular Biology Group
BSc, MSc, PhD, University of Melbourne
Dip Ed. La Trobe University
Current research interests investigate improved methods of vascularization (blood vessel growth) for tissue engineering and wound healing. This involves approaches that create human blood vessel networks in the laboratory for transplantation into animal models. Our group is further implementing this concept of pre-vascularization in a liver tissue engineering model where liver organoids are grown in the Laboratory for implantation into mice with liver disease. We are also examining if human endothelial cells demonstrate improved survival when implanted as multicellular spheroids rather than as single cells.
- Cao Y*, Mitchell G*, Messina A, Price L, Thompson E, Penington A, Morrison W, O’Connor A, Stevens G, Cooper- White JJ. The influence of architecture on degradation and tissue ingrowth into three dimensional Poly(Lactic-co-Glycolic Acid) scaffolds in vitro and in vivo. Biomaterials, 2006.27: 2854-64. 2.
- Lokmic Z, Darby IA, Thompson EW, Mitchell GM. A time course analysis of hypoxia, granulation tissue and blood vessel growth and remodelling in healing rat cutaneous incisional primary intention wounds. Wound Rep Regen, 2006, 14: 277-88.
- Lokmic Z, Stillaert F, Morrison WA, Thompson EW, Mitchell GM. An arterio-venous loop in a protected space generates a permanent, highly vascular, tissue engineered construct. FASEB J. 2007 21(2):511-522.
- Rophael JA, Craft RO, Palmer JA, Thomas GPL, Hussey AJ, Morrison WA, Penington AJ, GM Mitchell. Angiogenic Growth Factor Synergism in a Murine Tissue-Engineering Model of Angiogenesis and Adipogenesis. American Journal of Pathology, 2007, 171: 2048-2057.
- Lokmic Z and Mitchell GM. Engineering the microcirculation. Tissue Engineering, March 2008, 14B:87-103.
- Simcock JW, Penington AJ, Morrison WA, Thompson EW, and Mitchell GM. Endothelial precursor cells home to a vascularised tissue engineering chamber by application of the angiogenic chemokine CXCL12. Tissue Eng Part A. 2009,15 (3):655-64.
- Tilkorn D, Bedogni A, Keramidaris E, Han X, Palmer J, Dingle AM, Cowling BS, Williams MD, Mc Kay SM, Pepe L, Deftereos A, Morrison WA, Penington A, Mitchell GM. Implanted myoblast survival is dependent on the degree of vascularization in a novel delayed implantation /prevascularization tissue engineering model. Tissue Eng Part A. 2010 Jan;16(1):165-78.
- Lokmic Z, Mitchell GM, Visualization and stereological assessment of blood and lymphatic vessels. Histology and Histopathology, 2011 Jun;26(6):781-96
- Tilkorn DJ, Davies EM, Keramidaris E, Dingle AM, Gerrand Y-W, Taylor CJ, Han XL, Palmer JA, Penington AJ, Mitchell CA , Morrison WA, Dusting GJ, Mitchell GM In vitro myoblast preconditioning enhances subsequent survival post in vivo implantation into a tissue engineering chamber. Biomaterials, May, 2012, 33(15):3868-3879.
- Yap KK, Dingle AM, Palmer JA, Dhillon R, Lokmic Z, Penington AJ, Yeoh GC, Morrison WA, Mitchell GM, Enhanced liver progenitor cell survival and differentiation in vivo by spheroid implantation in a vascularized tissue engineering chamber, Biomaterials. 2013 May;34(16):3992-4001
- Taylor CJ, Church JE, Williams MD, Gerrand YW, Keramidaris E, Palmer JA, Galea LA, Penington AJ, Morrison WA, and Mitchell GM. Hypoxic preconditioning of myoblasts implanted in a tissue engineering chamber significantly increases local angiogenesis via upregulation of myoblast VEGF-A expression, and downregulation of miRNA-1, miRNA-206 and Angiopoietin 1. Journal of Tissue Engineering and Regenerative Medicine, 2018 Jan;12(1):e408-e421.
- Dingle AM*, Yap KK*, Gerrand Y-W, Taylor CJ, Keramidaris E, Lokmic Z, Kong AM, Peters HL, Morrison WA and Mitchell GM. Characterization of isolated liver sinusoidal endothelial cells for liver bio-engineering. Angiogenesis, 2018, 21(3), 581-597. *joint first authors.
- Yap KK, Yeoh GC, Morrison WA, Mitchell GM. The vascularised chamber as an in vivo bioreactor. Trends in Biotechnology, 2018 Oct;36(10):1011-1024.