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Analysers

SVI houses two 4-laser multi parameter LSR Fortessa instruments and a 3-Laser, 24 parameter Cytek Aurora spectral flow cytometer: They are both available 9am to 5pm Monday to Friday.

Comprehensive training is offered for instrument operation and a compulsory induction is required for first time users.

The current configuration of each instrument can be found in the table below, however there are alternative filters available to optimise specific experimental protocols.

Please contact Facility staff for assistance in filter arrangements to optimise your experiment.

 

 

Fortessa 1 - The Phantom

Fortessa 2 - Leela

Cytek Aurora

Laser Lines (nm)

405, 488, 561, 640

405, 488, 561, 640

405,488,640

Detection Channels

Detailed Table Here

Detailed Table Here

Detailed Table Here

96-Well HTS

Available

Not Available

Not Available

Sample Tube

5ml FACS Tube

96 well plate

5ml FACS Tube

5ml FACS Tube

Sample Concentration

1 x 106 cells / ml (200µl minimum volume)