The SVI Flow Cytometry Facility operates a 5-Laser BD Aria Fusion within a PC2 laboratory. The cell sorter is available 9am to 5pm Monday to Friday and will be operated by a member of staff. *Note: The cell sorter may be available outside these hours upon request.
The current configuration of the Cell Sorter can be found in the table below.
|
ARIA Fusion |
|
---|---|---|
Laser Lines (nm) |
355,405, 488, 561,640 |
|
Detection Channels |
||
Nozzle Size (µm) |
70, 85 100 |
|
Sample Tube |
5ml FACS Tube |
|
Collection Vessel |
4 x 5ml FACS Tube 2 x 15ml Falcon Tube 4 x 1.5ml Eppi Tube 6, 12, 48, 96 Well plate |
|
Sample Concentration |
Maximum 1 x 107 cells / ml (200µl minimum volume) |