Proteins are one of the body's most essential building blocks and are the molecular engines that control all functions of the body. Structural biology, including methods such as X-ray crystallography and cryo electron microscopy, offers the means to view the three-dimensional structure of proteins at the atomic level. Since the majority of drugs interact with proteins, atomic resolution structures have proved to be invaluable for the discovery and development of new drugs.
The work of the Structural Biology Unit is internationally recognised with the determination of more than one hundred crystal structures including those of membrane-associating proteins, detoxifying enzymes and protein kinases. This work has provided insights into a number of diseases such as cancer, bacterial and viral infections, and neurological diseases such as Alzheimer's disease. In recent years we have been emphasising the translational aspects of our work with an increasing focus on structure-based drug discovery. This focus has been underpinned by the development of virtual screening and fragment screening platforms in-house, by funding from the Australian Cancer Research Foundation, and partnerships with a number of Biotechnology companies including CSL Limited and Janssen.
The work undertaken by SVI scientists has provided major contributions to identification of targets for cancer therapy, and understanding the mechanisms of cancer growth and spread. This work was recognised in 2012 by a generous grant from the Australian Cancer Research Foundation to help establish the ACRF Rational Drug Discovery Centre at the Institute. Examples of the cancer projects currently being pursued by the Centre include:
GM-CSF and IL-3 receptors (with Profs Angel Lopez and Tim Hughes, Centre for Cancer Biology, Adelaide)
Cytokine receptors are transmembrane cell surface glycoproteins that bind specifically to cytokines and transduce their signals that can direct cells to proliferate, differentiate or even die. The GM-CSF, IL-3 and IL-5 family of cytokines regulates the survival, proliferation, differentiation and functional activation of hematopoietic cells. These same cytokines have also been implicated in multiple pathologies resulting from the excessive or aberrant expression of the cytokine or their receptors, in conditions such as certain types of leukaemia. We determined the structure of a GM-CSF:receptor ternary complex, representing the first structure of an "activated" receptor of this family of cytokines. Inspection of the structure revealed exciting insights into the mechanism of receptor activation and provided a unifying molecular explanation for the diverse functional properties of related cytokine:receptor systems. To maximise the drug development opportunities of this discovery we have formed a partnership with the biopharmaceutical company CSL Limited to discover and develop therapeutic antibodies that will disrupt aberrant signalling by the receptor. More recently we have determined the structure of the related IL-3 receptor bound to an antibody discovered by Angel Lopez’s lab and currently being tested in clinical trials for leukaemia treatment by CSL. The structure reveals how the antibody inhibits IL-3 signalling.
Glutathione transferases (with Prof Giorgio Ricci, University of Rome, Italy; Prof Paul Dyson, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland; Prof Philip Board, Australian National University, Canberra)
Glutathione S-transferases (GSTs) are a supergene family of enzymes that play a pivotal role in the detoxification of foreign chemicals and toxic metabolites. Paradoxically, the detoxifying activity of these enzymes is in part responsible for the development of cellular multi-drug resistance towards a number of chemotherapeutic agents. We have determined more than 50 GST crystal structures which have contributed to an understanding of the molecular basis of substrate recognition and catalysis by the enzyme superfamily. Inhibitors of GSTs have been used in clinical trials as adjuvants in cancer chemotherapy with some success. However, the use of these inhibitors has produced undesirable side effects and more suitable inhibitors are urgently being sought.
Mental illness encompasses a multitude of devastating conditions that present a major burden to the Australian economy. These conditions are often chronic and debilitating, posing the highest health, economic and social capital attrition burden to Australia of any disease group. We are pursuing a number of projects with aim of understanding how key proteins function in the brain with the ultimate aim of discovering new drugs to treat mental illness. Examples include:
Amyloid Precursor Protein (with Prof Colin Masters, Mental Health Research Institute and A/Prof Kevin Barnham, Melbourne University)
Alzheimer's disease is the most prevalent neurodegenerative disease in humans and is the fourth leading cause of death in the developed world. The disease is characterised by the presence of amyloid plaques that principally derive from amyloid precursor protein (APP). The long term aim of this project is to determine the complete structure of APP in order to understand its normal physiological function and as a basis for structure-based drug design of anti-Alzheimer's drugs. We are solving the structure of APP by a divide-and-conquer approach: solving the structures of small, overlapping fragments with the eventual aim of piecing the molecule together. To date we have determined the structures of three regions of APP: the growth factor domain, the copper-binding domain and the Abeta peptide bound to a range of clinically relevant antibodies.
IRAP (with Dr Siew Yeen Chai, Monash University)
Central administration of the hexapeptide angiotensin IV markedly enhances memory and learning in rodents. This effect is mediated by binding to a specific, high-affinity site in the brain which our collaborators identified to be the transmembrane enzyme, insulin-regulated aminopeptidase (IRAP). The peptide binds with high affinity to the catalytic domain of IRAP inhibiting its enzymatic activity. Using a molecular model of the catalytic domain, we have screened compound databases and have identified compounds that inhibit IRAP and reverse memory deficits in animals. To date, there is no proven effective treatment for cognitive impairment. Since the causes of cognitive impairment range from birth defects (Down's syndrome, mental retardation, cerebral palsy), recreational drug abuse, opportunistic infections to neurological conditions (stroke, brain trauma, neurodegeneration such as Alzheimer's disease), prevention therapies are not effective alternatives. The IRAP inhibitors may lead to the development of new classes of cognitive enhancers.
We have made major contributions to our understanding of how bacterial pore-forming toxins can pass through the walls of target cells. In recent years this work has expanded into studies of other infectious organisms such as parasites and viruses. Some examples of our work include:
Protein toxins (with Prof Rod Tweten, University of Oklahoma, USA; A/Prof Laurie Comstock, Harvard Medical School, USA)
Pore-forming toxins are promising model systems for understanding the biogenesis, structure and function of membrane channels as well as being potential targets for new antibiotics. One example of our work is perfringolysin O (PFO), a 52 kDa toxin secreted by the gas gangrene bacterium Clostridium perfringens, a member of a family of more than 20 toxins produced by Gram-positive bacteria. This family is often referred to as cholesterol-dependent cytolysins (CDCs) because of their strict requirement for membrane-bound cholesterol for activity. The presumed common mode of action of these toxins involves binding to target cell membranes via cholesterol, insertion into the lipid bilayer of target cells followed by oligomerisation and pore formation leading to cytolysis. However, the details of the molecular mechanism of membrane damage are not known. We have now determined the crystal structures of a number of CDCs. Current work revolves around understanding how CDC toxins penetrate membranes using X-ray crystallography, cryo electron microscopy and computational biology.